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Label retaining and stem cell marker expression in the developing rat urinary bladder.

Haiyang Zhang1, Guiting Lin, Xuefeng Qiu

  • 1Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143-0738, USA.

Urology
|December 27, 2011
PubMed
Summary
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This study used 5-ethynyl-2-deoxyuridine (EdU) labeling to track bladder stem cells in rats. Label retention did not correlate with common stem cell markers, suggesting c-kit expression relates to other cell types.

Area of Science:

  • Urology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Identifying stem cells is crucial for understanding tissue regeneration.
  • The urinary bladder's regenerative capacity suggests the presence of resident stem cells.
  • The label-retaining cell (LRC) strategy is a common method for identifying stem cells.

Purpose of the Study:

  • To identify potential stem cells in the urinary bladder using the LRC strategy.
  • To investigate the expression of putative stem cell markers in bladder cells.

Main Methods:

  • Newborn rats were injected with 5-ethynyl-2-deoxyuridine (EdU) to label dividing cells.
  • Bladders were harvested at various time points post-injection.
  • Immunofluorescence staining was performed for stem cell markers (Lgr5, CD34, SSEA-1, c-kit) and EdU.

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Main Results:

  • EdU-labeled cells decreased over time, with initial labeling in both epithelial and detrusor compartments.
  • Label retention was random and not associated with Lgr5, CD34, or SSEA-1 expression.
  • A significant overlap was observed between EdU-positive cells and c-kit expression, particularly in the lamina propria and detrusor.

Conclusions:

  • Label retention in the bladder does not appear to be associated with Lgr5, CD34, or SSEA-1.
  • The observed association of label retention with c-kit suggests these cells are likely interstitial cells of Cajal, not stem cells.
  • Further research is needed to definitively identify bladder stem cells.