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12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
Interleukin-17A increases leptin production in human bone marrow mesenchymal stem cells
1College of Pharmacy, Ajou University, Suwon, Gyeounggi-do 443-749, Republic of Korea. minsoo@alum.mit.edu
Biochemical Pharmacology
|December 27, 2011
Summary
Interleukin-17A (IL-17A) promotes osteogenesis and inhibits adipogenesis in human bone marrow mesenchymal stem cells (hBM-MSCs) by increasing leptin production. This leptin mediation is key to understanding stem cell lineage commitment and bone metabolism in diseases like type II diabetes.
Area of Science:
- Stem cell biology
- Endocrinology
- Metabolic bone disease
Background:
- Human bone marrow mesenchymal stem cells (hBM-MSCs) are a model for studying type II diabetes and bone metabolism.
- Leptin and IL-17A inversely regulate adipogenesis and osteogenesis in MSCs.
- IL-17A's role in metabolic and bone diseases necessitates understanding its mechanism in MSC lineage commitment.
Purpose of the Study:
- To investigate if IL-17A's inverse regulation of adipogenesis and osteogenesis in hBM-MSCs is linked to endogenous leptin production.
- To elucidate the molecular mechanisms underlying IL-17A-mediated lineage commitment in hBM-MSCs.
Main Methods:
- Analysis of adiponectin and leptin secretion profiles in hBM-MSCs under various differentiation conditions.
- Treatment with IL-17A and anti-leptin antibodies.
- Assessment of adipogenesis and osteogenesis markers.
Main Results:
- Dexamethasone increased leptin production in hBM-MSCs, with higher levels during osteogenesis than adipogenesis.
- IL-17A significantly increased leptin production in hBM-MSCs, particularly under osteogenic conditions.
- Anti-leptin antibody partially blocked IL-17A's inhibition of adipogenesis, indicating leptin's mediating role.
Conclusions:
- IL-17A-induced leptin production is a key factor in the inverse regulation of adipogenesis and osteogenesis in hBM-MSCs.
- Leptin produced in extra-adipose tissues, like MSCs and osteoblasts, plays a significant role in metabolic and bone diseases.
- Understanding this pathway offers insights into type II diabetes and abnormal bone metabolism.
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