Microbacterium murale sp. nov., isolated from an indoor wall
P Kämpfer1, J Schäfer1, N Lodders1
1Institut für AngewandteMikrobiologie, Justus-Liebig-Universität Giessen, Ludwigstrasse 23, D-35392 Giessen, Germany.
International Journal of Systematic and Evolutionary Microbiology
|December 27, 2011
Summary
A novel bacterial species, Microbacterium murale sp. nov., was identified from a mould-colonized wall. This Gram-positive rod exhibits distinct phylogenetic and biochemical characteristics, differentiating it from known Microbacterium species.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Microbacterium species are known for their presence in diverse environments.
- Identification of novel bacteria is crucial for understanding microbial diversity and function.
Purpose of the Study:
- To characterize and classify a novel bacterial isolate, designated 01-Gi-001(T), from a mould-colonized wall.
- To determine the phylogenetic and chemotaxonomic properties of the novel isolate.
Main Methods:
- 16S rRNA gene sequence analysis for phylogenetic placement.
- Determination of peptidoglycan diamino acid, menaquinones, polar lipids, and fatty acid profiles.
- Physiological and biochemical characterization, including DNA-DNA relatedness studies.
Main Results:
- Strain 01-Gi-001(T) belongs to the genus Microbacterium, with highest 16S rRNA gene sequence similarity to M. hydrocarbonoxydans (98.9%).
- Diagnostic diamino acid (ornithine), major menaquinones (MK-13, MK-12), polar lipids, and fatty acids (anteiso-C15:0, iso-C16:0, anteiso-C17:0) were identified.
- Distinct physiological, biochemical, and DNA-DNA relatedness values confirmed its novelty.
Conclusions:
- Strain 01-Gi-001(T) represents a novel species within the genus Microbacterium.
- The novel species is proposed as Microbacterium murale sp. nov., with type strain 01-Gi-001(T) (=DSM 22178(T)=CCM 7640(T)).
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