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Updated: May 26, 2026
The In ovo CAM-assay as a Xenograft Model for Sarcoma
Published on: July 17, 2013
Establishing efficient xenograft models with intrinsic vascularisation for growing primary human low-grade sarcomas
Daniel Tilkorn1, Adrien Daigeler, Ingo Stricker
1Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, 44789 Bochum, North Rhine Westphalia, Germany. d.tilkorn@web.de
Background:
There are no xenograft models of low-grade soft tissue sarcoma. Transplant survival remains an obstacle in sarcoma xenograft models and is attributed to post-transplantation hypoxia. Models with an intrinsic tissue - engineered vascular supply may overcome this obstacle. The aim of this study was to establish a novel xenograft model of primary human low grade soft tissue sarcoma.
Materials And Methods:
Primary low-grade liposarcoma fragments were transplanted into a silicon chamber, placed around the superficial epigastric vessels in athymic nude mice. Xenograft samples were assessed histologically (light/electron microscopy and immunohistochemistry for S100).
Results:
All xenotransplants of low grade primary soft tissue liposarcoma (n=4) engrafted, led to the development of solid tumours in mice. Histological and immunohistochemical staining confirmed the xenografts as being well-differentiated liposarcomas identical to the original tumor tissue.
Conclusion:
Successful transplantation of human low-grade liposarcoma tissue in mice was established for the first time using a model with an intrinsic vascular supply.
