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Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

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NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
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Published on: June 4, 2021

A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR.

Ching-Yu Chou1, Minglee Chu, Chi-Fon Chang

  • 1Department of Physics, National Taiwan Normal University, Taipei 116, Taiwan, ROC.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|December 28, 2011
PubMed
Summary

Researchers developed a novel NMR sample shuttle for enhanced molecular dynamics analysis. This device enables low-field relaxation measurements, improving macromolecular function understanding.

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Area of Science:

  • Biophysics
  • Structural Biology
  • Nuclear Magnetic Resonance (NMR) Spectroscopy

Background:

  • NMR relaxation analysis offers insights into molecular dynamics and macromolecular functions.
  • Traditional NMR methods are limited to fixed high fields, restricting comprehensive dynamic information extraction.

Purpose of the Study:

  • To design and evaluate a compact, high-speed servo-mechanical shuttle for NMR sample manipulation.
  • To enable NMR relaxation measurements at low magnetic fields.

Main Methods:

  • Developed a servo-mechanical shuttle assembly for a 600 MHz superconducting magnet.
  • The shuttle transports NMR samples between high and low magnetic fields rapidly (100 ms).
  • Acquired 2D-(15)N-HSQC spectra of ubiquitin and determined field-dependent (13)C-R(1) data for alanine.

Main Results:

  • The shuttle achieved rapid sample transfer with minimal sensitivity loss.
  • Demonstrated utility for R(1) measurements of macromolecules at low fields.
  • Analyzed contributions of chemical shift anisotropy (CSA) and (1)H-(13)C dipolar interactions to carboxyl (13)C-R(1).

Conclusions:

  • The developed NMR shuttle significantly expands the capabilities for studying molecular dynamics.
  • Facilitates more complete dynamic information extraction by enabling low-field relaxation measurements.
  • Provides a valuable tool for biophysical and structural biology research.