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Related Experiment Video

Updated: May 26, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Antibody microarrays for label-free cell-based applications.

Sarah Milgram1, Radoslaw Bombera, Thierry Livache

  • 1CREAB Group, SPRAM Laboratory UMR 5819 (CEA-CNRS-UJF Grenoble 1), Institute for Nanosciences and Cryogeny, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble, France.

Methods (San Diego, Calif.)
|December 28, 2011
PubMed
Summary

This study presents three novel chemical methods for fabricating antibody microarrays for cell analysis using Surface Plasmon Resonance imaging (SPRi). These techniques enable efficient, label-free, and real-time detection of cellular activity and characterization.

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Area of Science:

  • Biotechnology
  • Microtechnology
  • Analytical Chemistry

Background:

  • Microarrays are increasingly used for cell analysis, requiring specific solid supports for various detection techniques.
  • Real-time, label-free methods like Surface Plasmon Resonance imaging (SPRi) are gaining interest for miniaturized cell analysis platforms.

Purpose of the Study:

  • To develop and evaluate chemical fabrication methods for antibody microarrays tailored for SPRi-based cell analysis.
  • To create versatile microarray platforms for specific cell capture, controlled release, and simultaneous characterization of cell type and secretory activity.

Main Methods:

  • Developed three distinct chemical approaches for antibody microarray fabrication: polypyrrole-based chemistry for cell capture, a three-level assembly (polypyrrole-DNA-antibody) for cell release, and thiol-based chemistry for complex samples.

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Last Updated: May 26, 2026

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Published on: November 23, 2015

  • Utilized Surface Plasmon Resonance imaging (SPRi) for label-free, real-time detection and analysis of cellular samples on the fabricated microarrays.

    • Main Results:

    • Successfully fabricated antibody microarrays capable of specific whole living cell capture using polypyrrole chemistry.
    • Demonstrated controlled cell release after capture by employing a DNA and antibody conjugate assembly.
    • Developed a thiol-based chemistry approach enabling simultaneous cell type characterization and detection of secreted proteins in complex biological samples.

    Conclusions:

    • The study introduces three original, efficient methods for on-chip immunoaffinity-based cell analysis using microarrays.
    • These novel fabrication techniques facilitate rapid and versatile analysis of cellular samples, advancing miniaturized cell analysis platforms.