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Related Experiment Video

Updated: May 26, 2026

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

Inflammation-induced lymph node lymphangiogenesis is reversible.

Viviane Mumprecht¹, Filip Roudnicky¹, Michael Detmar¹

¹Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland.

The American Journal of Pathology

|December 28, 2011

Summary

Lymph node lymphangiogenesis, an early sign of metastasis, can be imaged using anti-LYVE-1 immuno-PET. This study confirms that inflammation-induced lymphatic vessel expansion in lymph nodes regresses upon inflammation resolution.

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Area of Science:

Oncology
Immunology
Medical Imaging

Background:

Lymph node metastasis extent predicts cancer progression.
Current noninvasive imaging lacks sensitivity for early metastasis detection.
Lymphangiogenesis in lymph nodes may be an early indicator of metastasis.

Purpose of the Study:

To investigate if inflammation-induced lymph node lymphangiogenesis resolves after inflammation subsides.
To determine if anti-LYVE-1 immuno-PET can detect the regression of lymphatic vessels.
To differentiate between inflammation-induced and metastasis-induced lymphangiogenesis.

Main Methods:

Developed and utilized anti-LYVE-1 immuno-PET for imaging lymph node lymphangiogenesis in mice.
Established a mouse model of skin inflammation to study lymphatic vessel changes.
Monitored lymphatic network changes in lymph nodes during and after inflammation resolution.

Main Results:

Lymphatic vessels in tumor-draining lymph nodes showed regression upon resolution of inflammation.
Anti-LYVE-1 immuno-PET successfully visualized the regression of lymphatic networks.
Demonstrated that inflammation-induced lymphangiogenesis is reversible.

Conclusions:

Lymph node lymphangiogenesis induced by inflammation is a transient process.
Anti-LYVE-1 immuno-PET is a sensitive tool for detecting lymph node lymphangiogenesis and its regression.
This finding is crucial for accurately identifying lymph node metastasis in cancer patients.