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Related Experiment Video

Updated: May 26, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

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Dnmt3a silences hematopoietic stem cell self-renewal.

Jennifer J Trowbridge¹, Stuart H Orkin

¹Department of Pediatric Oncology, Dana-Farber Cancer Institute, Division of Hematology/Oncology, Children's Hospital Boston, Boston, Massachusetts, USA. trowbridge@bloodgroup.tch.harvard.edu

Nature Genetics

|December 28, 2011

Summary

DNA methylation by Dnmt3a silences self-renewal genes in hematopoietic stem cells (HSCs). This epigenetic regulation is crucial for enabling HSCs to differentiate effectively during development.

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Area of Science:

Epigenetics
Developmental Biology
Hematopoiesis

Background:

DNA methylation is a key epigenetic mechanism controlling gene expression during development.
Hematopoietic stem cells (HSCs) possess self-renewal and differentiation capacities crucial for blood formation.

Purpose of the Study:

To investigate the role of DNA methyltransferase 3a (Dnmt3a) in regulating HSC self-renewal and differentiation.
To understand the epigenetic control of hematopoietic stem cell fate.

Main Methods:

Analysis of Dnmt3a function in hematopoietic stem cells.
Investigating gene expression patterns related to self-renewal and differentiation.

Main Results:

Dnmt3a acts as a DNA methyltransferase essential for silencing genes associated with HSC self-renewal.
Dnmt3a-mediated silencing of self-renewal genes permits efficient hematopoietic differentiation.

Conclusions:

Dnmt3a plays a critical role in epigenetic reprogramming of hematopoietic stem cells.
Targeting Dnmt3a may offer therapeutic strategies for modulating HSC differentiation and treating hematologic disorders.