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Updated: May 26, 2026

Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
The association of human primary bone cells with biphasic calcium phosphate (βTCP/HA 70:30) granules increases bone
Ronaldo de Oliveira Lomelino1, Igor Iuco Castro-Silva, Adriana Brandão Ribeiro Linhares
1Post-Graduation Program on Medical Sciences, Fluminense Federal University (UFF), Niteroi, RJ, Brazil.
Abstract:
This work evaluates the suitability of biphasic calcium phosphate (BCP) granules (β-TCP/HA 70:30) as potential carriers for cell-guided bone therapy. The BCP granules were obtained by synthesis in the presence of wax, thermal treatment, crushing and sieving and characterized by scanning electron microscopy (SEM), X-ray diffraction and Fourier transform infrared spectroscopy. The cytocompatibility of the BCP granules was confirmed by a multiparametric cytotoxicity assay. SEM analysis showed human bone cell adhesion and migration after seeding onto the material. Rat subcutaneous xenogeneic grafting of granules associated to human bone cells revealed a more accentuated moderate chronic inflammatory infiltrate, without signs of a strong xenoreactivity. Histomorphometrical analysis of bone repair of defects in rat skulls (∅ = 5 mm) has shown that bone cell associated-BCP and autograft promoted a two- and threefold increase, respectively, on new bone formation after 45 days, as compared to BCP alone and blood clot. The increase in bone repair supports the suitability the biocompatible (70:30) BCP granules as injectable and mouldable scaffolds for human cells in bone bioengineering.
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