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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

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Published on: October 1, 2009

Polyamines analysis by HPLC and their application as tumor markers.

Shanji Fu¹, Chen Xiao, Weiming Zhao

¹Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China.

Frontiers in Bioscience (Elite Edition)

|December 29, 2011

Summary

Polyamines like putrescine, spermidine, and spermine show promise as cancer biomarkers. A new HPLC method efficiently measures these tumor markers in human serum, aiding cancer diagnosis and monitoring.

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Area of Science:

Biochemistry
Oncology
Analytical Chemistry

Background:

Cancers represent a leading global cause of mortality, driving the need for effective diagnostic markers.
Polyamines, including putrescine (PUT), spermidine (SPD), and spermine (SP), are frequently elevated in cancer patients, suggesting their potential as tumor markers.

Purpose of the Study:

To develop and validate an efficient high-performance liquid chromatographic (HPLC) method for quantifying polyamines in human serum.
To evaluate the diagnostic potential of polyamines as cancer biomarkers compared to 24 established tumor markers.

Main Methods:

Development of a rapid HPLC method utilizing dansyl chloride for pre-column derivatization of polyamines.
Serum samples from patients with 11 different cancer types were analyzed.
Comparative analysis of polyamines against 24 common tumor markers using specificity, sensitivity, and predictive values.

Main Results:

The developed HPLC method achieved separation of polyamines within 10 minutes, demonstrating simplicity, speed, and reproducibility.
Polyamines exhibited promising performance across multiple diagnostic metrics when compared to existing tumor markers.

Conclusions:

Polyamines are identified as valuable potential tumor markers.
These biomarkers may significantly contribute to cancer diagnosis, prediction of treatment efficacy, and monitoring of tumor recurrence.