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Double protein functionalized poly-ε-caprolactone surfaces: in depth ToF-SIMS and XPS characterization.

T Desmet¹, C Poleunis, A Delcorte

¹Polymer Chemistry & Biomaterials Research Group, Ghent University, Ghent, Belgium.

Journal of Materials Science. Materials in Medicine

|December 29, 2011

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Researchers enhanced poly-ε-caprolactone (PCL) biomaterial cell adhesion by grafting 2-aminoethyl methacrylate (AEMA) and immobilizing gelatin and fibronectin proteins. Advanced surface analysis confirmed successful modifications, even after sterilization.

Area of Science:

Biomaterials Science
Surface Chemistry
Polymer Science

Background:

Biomaterial surface modification is crucial for enhancing cell adhesion.
Poly-ε-caprolactone (PCL) is a widely used biomaterial.
Previous work established a method for PCL functionalization with 2-aminoethyl methacrylate (AEMA).

Purpose of the Study:

To comprehensively characterize PCL surfaces functionalized with AEMA and subsequently coated with gelatin and fibronectin.
To validate the effectiveness of Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and X-ray Photoelectron Spectroscopy (XPS) for surface analysis.
To assess the impact of ethylene oxide sterilization on the modified surfaces.

Main Methods:

Surface functionalization of PCL films via Ar-plasma treatment and UV-initiated AEMA grafting.

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Covalent immobilization of gelatin followed by fibronectin physisorption.

In-depth surface analysis using XPS and ToF-SIMS.

Main Results:

XPS and ToF-SIMS confirmed homogeneous AEMA grafting and successful protein coating.
ToF-SIMS identified polymethacrylates and specific amino acid-related ions, distinguishing between gelatin and fibronectin.
XPS analysis of C- and N-peaks confirmed protein presence.
Ethylene oxide sterilization did not alter the surface chemical composition.

Conclusions:

XPS and ToF-SIMS are complementary techniques for detailed characterization of biomaterial surface modifications.
The developed surface modification strategy effectively immobilizes proteins, enhancing biomaterial properties.
The modified surfaces exhibit stability towards common sterilization methods, indicating potential for biomedical applications.