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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Sequential multiplexed analyte quantification using peptide immunoaffinity enrichment coupled to mass spectrometry.

Jeffrey R Whiteaker1, Lei Zhao, Chenwei Lin

  • 1Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.

Molecular & Cellular Proteomics : MCP
|December 29, 2011
PubMed
Summary

Peptide immunoaffinity enrichment coupled to selected reaction monitoring mass spectrometry (immuno-SRM) enables multiplex analysis of numerous peptides. This quantitative proteomic technique demonstrates high accuracy and reproducibility for large-scale biomarker verification and pathway analysis.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Peptide immunoaffinity enrichment coupled to selected reaction monitoring mass spectrometry (immuno-SRM) is a promising quantitative proteomic technology.
  • A key advantage of immuno-SRM is its potential for multiplex analysis, allowing concurrent quantification of multiple analytes.

Purpose of the Study:

  • To explore the capacity of immuno-SRM for analyzing large numbers of analytes.
  • To evaluate the multiplex capabilities of immuno-SRM.
  • To demonstrate the sequential analysis of peptide groups from a single sample.

Main Methods:

  • Immuno-SRM assays were configured in multiplex groups of 10, 20, 30, 40, and 50 peptides using common reagents.
  • Synthetic peptides were measured in plasma across several orders of magnitude concentration.
  • Sequential enrichment of analyte peptide sets was performed on plasma samples.

Main Results:

  • Multiplex immuno-SRM assays showed high correlation (r(2) ≥ 0.98) and good agreement (bias ≤ 1%) with single-plex assays.
  • Sequential enrichment of peptide groups demonstrated good agreement (bias ≤ 1.5%) and consistent reproducibility.
  • The technique effectively measures peptides across a wide concentration range.

Conclusions:

  • Immuno-SRM is highly effective for multiplex analysis of numerous analytes.
  • The technology supports sequential enrichment strategies for complex sample analysis.
  • Immuno-SRM is valuable for large-scale biomarker verification and targeted pathway analysis.