Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of upstream priming on transient downstream platelet-substrate interactions.

Colloids and surfaces. B, Biointerfaces·2021

Same author

Silica Nanoparticle-Endothelial Interaction: Uptake and Effect on Platelet Adhesion under Flow Conditions.

ACS applied bio materials·2021

Same author

Microfluidic assay of antiplatelet agents for inhibition of shear-induced platelet adhesion and activation.

Lab on a chip·2020

Same author

Effects of elapsed time on downstream platelet adhesion following transient exposure to elevated upstream shear forces.

Colloids and surfaces. B, Biointerfaces·2020

Same author

Downstream platelet adhesion and activation under highly elevated upstream shear forces.

Acta biomaterialia·2019

Same author

Effects of upstream shear forces on priming of platelets for downstream adhesion and activation.

Acta biomaterialia·2018

Search research articles

Related Experiment Video

Updated: May 26, 2026

Microcontact Printing of Proteins for Cell Biology

Microcontact Printing of Proteins for Cell Biology

Published on: December 5, 2008

Multiprotein microcontact printing with micrometer resolution.

Colin D Eichinger¹, Tony W Hsiao, Vladimir Hlady

¹Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA.

Langmuir : the ACS Journal of Surfaces and Colloids

|December 30, 2011

Summary

This summary is machine-generated.

This study introduces a multiprotein microcontact printing (microCP) technique for precise protein patterning. This method enables the creation of complex protein arrangements crucial for tissue engineering and regenerative medicine applications.

More Related Videos

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Published on: October 31, 2014

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Related Experiment Videos

Last Updated: May 26, 2026

Microcontact Printing of Proteins for Cell Biology

Microcontact Printing of Proteins for Cell Biology

Published on: December 5, 2008

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Published on: October 31, 2014

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Area of Science:

Biomaterials Science
Cell Biology
Tissue Engineering

Background:

Precise spatial arrangement of multiple proteins is vital for mimicking natural cellular environments.
Existing techniques may lack the resolution or flexibility for complex multiprotein patterning.

Purpose of the Study:

To develop and validate a multiprotein microcontact printing (microCP) technique.
To achieve micrometer precision in aligning multiple protein patterns sequentially.

Main Methods:

Fabrication of soft polymeric stamps.
Sequential protein printing using an inverted microscope for real-time visualization and alignment.
Utilizing a specialized microscope stage and objective setup for precise control.

Main Results:

Demonstrated successful sequential printing of multiple proteins with micrometer precision.
Achieved accurate registration and alignment of protein patterns.
Observed astrocyte cells interacting with and aligning along the printed protein lanes.

Conclusions:

The developed multiprotein microCP technique offers high precision for creating biomimetic protein patterns.
This technique is a valuable tool for advancing tissue engineering and regenerative medicine.
The ability to control protein placement influences cell behavior and alignment.