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HDL-associated enzymes and proteins in hemodialysis patients

Leonardo D Cacciagiú1, Ana I González, Leonardo Gomez Rosso

  • 1Laboratory of Lipids and Lipoproteins, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Junín 954, CP1113AAD, Buenos Aires, Argentina.

Clinical Biochemistry
|December 31, 2011
PubMed

Insights

High-density lipoprotein (HDL) functionality, not just concentration, is crucial for hemodialysis patients. HDL-associated proteins and enzymes are altered, impacting lipid profiles and inflammation in chronic kidney disease.

Area of Science:

  • Biochemistry
  • Nephrology
  • Cardiovascular Research

Background:

  • Chronic kidney disease (CKD) patients on hemodialysis exhibit dyslipidemia and chronic inflammation.
  • High-density lipoprotein (HDL) plays a critical role in reverse cholesterol transport and possesses anti-inflammatory and antioxidant properties.

Purpose of the Study:

  • To investigate HDL-associated proteins and enzymes in hemodialysis patients.
  • To determine the relationship between these HDL components, lipoprotein profiles, and inflammatory markers.

Main Methods:

  • Cross-sectional study comparing 53 hemodialysis patients with 32 healthy controls.
  • Assessed plasma lipids, apolipoprotein-AI (Apo-AI), and high-sensitivity C-reactive protein (hs-CRP).
  • Measured activities of cholesteryl ester transfer protein (CETP), paraoxonase, lipoprotein lipase A2 (LpPLA2), and postheparin hepatic lipase.

Main Results:

  • Hemodialysis patients had higher triglycerides and lower LDL-, HDL-, and total cholesterol compared to controls.
  • Apo-AI, paraoxonase, and hepatic lipase levels were significantly lower in patients.
  • CETP levels were higher in patients, while LpPLA2 showed no significant difference between groups.

Conclusions:

  • Lipoprotein metabolism is significantly altered in hemodialysis patients.
  • HDL functionality, influenced by its associated proteins and enzymes, is impaired.
  • These alterations may contribute to the pro-oxidative and pro-inflammatory state observed in CKD.
Abstract

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