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Related Concept Videos

Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

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Related Experiment Video

Updated: May 26, 2026

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)
10:28

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

Published on: May 5, 2023

DNA replication, RNAi and epigenetic inheritance.

Marlyn Gonzalez1, Fei Li

  • 1Department of Biology, New York University, New York, NY, USA.

Epigenetics
|December 31, 2011
PubMed
Summary
This summary is machine-generated.

DNA polymerase subunit Cdc20 is crucial for maintaining histone methylation and heterochromatin during DNA replication. It coordinates RNA interference and histone modification, ensuring epigenetic inheritance in fission yeast.

Related Experiment Videos

Last Updated: May 26, 2026

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)
10:28

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

Published on: May 5, 2023

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genetics

Background:

  • Histone methylation is vital for chromatin structure and gene expression.
  • Faithful inheritance of epigenetic marks during DNA replication remains poorly understood.
  • H3K9 methylation is a key heterochromatin mark in fission yeast, regulated by RNA interference (RNAi).

Purpose of the Study:

  • To investigate the role of DNA polymerase components in the inheritance of histone methylation.
  • To elucidate the mechanism by which epigenetic marks are transmitted across cell divisions.
  • To understand the coordination between DNA replication, RNAi, and histone modification.

Main Methods:

  • Utilized fission yeast as a model organism.
  • Investigated the association of DNA polymerase subunit Cdc20 with silencing factors.
  • Assessed the impact of Cdc20 on H3K9 methylation, heterochromatin function, and siRNA generation.

Main Results:

  • Identified Cdc20, a DNA polymerase subunit, as essential for H3K9 methylation and heterochromatin.
  • Demonstrated that Cdc20 associates with the Dos2-Rik1 silencing complex.
  • Showed Cdc20 promotes RNA polymerase II transcription of heterochromatin, regulating siRNA generation.

Conclusions:

  • DNA polymerase components, specifically Cdc20, play a critical role in epigenetic inheritance.
  • Cdc20 coordinates DNA replication, RNAi, and histone methylation for faithful transmission of H3K9 methylation.
  • Propose a model where DNA polymerase subunits recruit epigenetic factors at replication forks to ensure heterochromatin stability.