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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

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Updated: May 26, 2026

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

[Application of next generation sequencing techniques in plant transcriptome].

Ye Liang¹, Shuang-Yan Chen, Gong-She Liu

¹Institute of Bontany, Chinese Acdemy of Sciences, Beijing, China. hawkliangsu27@sina.com

Yi Chuan = Hereditas

|December 31, 2011

Summary

Next-generation sequencing (NGS) offers high-throughput, low-cost DNA sequencing for biological research. This review uses the 454 platform to explore NGS principles, plant transcriptome applications, and future directions.

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Area of Science:

Molecular Biology
Genomics
Bioinformatics

Context:

DNA sequencing technologies have advanced significantly.
Next-generation sequencing (NGS) provides high-throughput and cost-effective data generation.
The 454 sequencing platform is a mature and widely adopted NGS technology.

Purpose:

To explain the technical principles of NGS, highlighting advantages and disadvantages.
To review current applications of NGS in plant transcriptome research.
To forecast future developments and applications of NGS in plant science.

Summary:

This paper examines Next-Generation Sequencing (NGS) techniques, using the 454 platform as a case study.
It details the principles, benefits, and limitations of NGS technologies.
Applications in plant transcriptome analysis are reviewed, with future prospects discussed.

Impact:

Provides researchers with a comprehensive overview of NGS for biological studies.
Highlights the utility of NGS in advancing plant science and transcriptome research.
Informs future research directions and technological development in plant genomics.