Human serum protein adsorption onto synthesis nano-hydroxyapatite

Area of Science:

• Biomaterials Science
• Surface Chemistry
• Biochemistry

Background:

• Nano-hydroxyapatite (HA) is a key biomaterial with potential applications in diagnostics and therapeutics.
• Understanding protein adsorption onto HA is crucial for designing biocompatible materials and optimizing biosensor performance.
• Human serum proteins (HSP), including albumin and total protein, are primary interactants with biomaterials in vivo.

Purpose of the Study:

• To investigate the adsorption behavior of human serum proteins (HSP) onto synthesized nano-hydroxyapatite (HA).
• To determine the most suitable adsorption isotherm model for describing HSP-HA interactions.
• To evaluate the influence of temperature on the adsorption of HSP onto nano-HA.

Main Methods:

• Synthesized high-purity nano-hydroxyapatite (Ca₁₀(PO₄)₆(OH)₂).
• Studied adsorption of human serum proteins (albumin and total protein) using UV-visible spectrophotometry across a range of temperatures.
• Fitted experimental adsorption data to Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich (DR) isotherm models.

Main Results:

• The Dubinin-Radushkevich (DR) isotherm model provided the best fit for the experimental adsorption data.
• Increased temperature generally led to enhanced adsorption of human serum proteins onto the nano-HA.
• The observed temperature dependence is attributed to increased HSP activity and diffusion rates on the HA surface.

Conclusions:

• The DR isotherm model accurately describes HSP adsorption onto nano-HA.
• Temperature plays a significant role in modulating HSP adsorption onto nano-HA, with higher temperatures favoring increased adsorption.
• These findings provide valuable insights for the development of HA-based biomedical devices and drug delivery systems.