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Updated: May 26, 2026

The Use of the Puzzle Box as a Means of Assessing the Efficacy of Environmental Enrichment
Published on: December 29, 2014
Environmental enrichment decreases GABAergic inhibition and improves cognitive abilities, synaptic plasticity, and
Tatjana Begenisic1, Maria Spolidoro, Chiara Braschi
1Laboratory of Neurobiology, Scuola Normale Superiore Pisa, Italy.
Abstract:
Down syndrome (DS) is the most common genetic disorder associated with mental retardation. It has been repeatedly shown that Ts65Dn mice, the prime animal model for DS, have severe cognitive and neural plasticity defects due to excessive inhibition. We report that increasing sensory-motor stimulation in adulthood through environmental enrichment (EE) reduces brain inhibition levels and promotes recovery of spatial memory abilities, hippocampal synaptic plasticity, and visual functions in adult Ts65Dn mice.

