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Related Experiment Video

Updated: May 26, 2026

Flash Freezing and Cryosectioning E12.5 Mouse Brain
28:32

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Published on: May 28, 2007

[Cryosurgery]

Katsuto Shinohara1

  • 1Department of Urology, University of California, San Francisco, USA.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|January 3, 2012
PubMed
Summary

No abstract available in PubMed .

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Flash Freezing and Cryosectioning E12.5 Mouse Brain
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Published on: May 28, 2007

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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