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Reduced expression of tissue factor pathway inhibitor-2 contributes to apoptosis and angiogenesis in cervical cancer

Qiao Zhang1, Yao Zhang, Shi Z Wang

  • 1Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.

Journal of Experimental & Clinical Cancer Research : CR
|January 3, 2012
PubMed
Abstract

Insights

Tissue factor pathway inhibitor-2 (TFPI-2) expression decreases with cervical cancer progression. Lower TFPI-2 levels correlate with increased tumor cell apoptosis and angiogenesis, suggesting an inhibitory role in cervical cancer development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Tissue factor pathway inhibitor-2 (TFPI-2) is a Kunitz-type serine proteinase inhibitor associated with the extracellular matrix.
  • Downregulation of TFPI-2 has been implicated in tumor invasion and metastasis in various cancers.

Purpose of the Study:

  • To investigate the expression of TFPI-2 in normal cervical squamous epithelia, cervical intraepithelial neoplasia (CIN), and cervical cancer.
  • To determine the correlation between TFPI-2 expression and clinicopathological features, including tumor stage, lymph node metastasis, HPV infection, apoptosis, and angiogenesis.

Main Methods:

  • Immunohistochemistry was used to assess the expression of TFPI-2, Ki-67, and vascular endothelial growth factor (VEGF).
  • Apoptotic index (AI) was determined using an in situ end-labeling assay (TUNEL).
  • Microvessel density (MVD) was evaluated using CD34 staining.

Main Results:

  • TFPI-2 expression showed a decreasing trend with cervical cancer progression and was significantly correlated with FIGO stage, lymph node metastasis, and HPV infection.
  • TFPI-2 expression positively correlated with AI (P=0.004) and negatively correlated with VEGF and MVD (p < 0.001).
  • No significant correlation was found between Ki-67 and TFPI-2 expression in cervical cancer.

Conclusions:

  • TFPI-2 expression decreases as cervical cancer progresses.
  • TFPI-2 expression is closely associated with tumor cell apoptosis and angiogenesis in cervical cancer.
  • TFPI-2 may exert an inhibitory effect on the development of cervical cancer.

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