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Related Experiment Video

Updated: May 26, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

Cell-targeting fusion constructs containing recombinant gelonin.

Mi-Ae Lyu¹, Yu Joshua Cao, Khalid A Mohamedali

¹Immunopharmacology and Targeted Therapy Laboratory, Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston, Texas, USA.

Methods in Enzymology

|January 3, 2012

Summary

Novel immunotoxins combining gelonin toxin with growth factors or antibodies show potent and selective targeting of cancer cells. These agents demonstrate significant potential as therapeutic candidates for both solid tumors and hematological malignancies in clinical trials.

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Last Updated: May 26, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo

Published on: January 4, 2012

Area of Science:

Biotechnology and Pharmaceutical Sciences
Oncology
Molecular Biology

Background:

Therapeutic agents targeting tumor cells, including immunotoxins, have shown promise in clinical trials for various cancers.
Established tumors and micrometastases are key targets for effective cancer therapies.

Purpose of the Study:

To design and characterize novel recombinant gelonin toxin-based immunotoxins.
To evaluate the efficacy of these constructs against solid tumors, tumor vasculature, and hematological malignancies.

Main Methods:

Fusion of recombinant gelonin toxin with growth factors or single-chain antibodies.
In vitro and in vivo characterization of construct cytotoxicity, selectivity, and specificity.

Main Results:

Constructs exhibited cytotoxicity at nanomolar or sub-nanomolar levels.
Demonstrated impressive selectivity and specificity for antigen-bearing target cells.
Successful targeting of solid tumor cells, tumor vasculature, and hematological malignancies.

Conclusions:

The designed immunotoxin constructs are potent and selective therapeutic agents.
These agents show significant potential as candidates for clinical trials in oncology.
Further development of these targeted toxins could lead to improved cancer treatments.