Related Experiment Video

Updated: May 26, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

Cell-targeting fusion constructs containing recombinant gelonin

Mi-Ae Lyu1, Yu Joshua Cao, Khalid A Mohamedali

  • 1Immunopharmacology and Targeted Therapy Laboratory, Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston, Texas, USA.

Methods in Enzymology
|January 3, 2012
PubMed

Insights

Novel immunotoxins combining gelonin toxin with growth factors or antibodies show potent and selective targeting of cancer cells. These agents demonstrate significant potential as therapeutic candidates for both solid tumors and hematological malignancies in clinical trials.

Area of Science:

  • Biotechnology and Pharmaceutical Sciences
  • Oncology
  • Molecular Biology

Background:

  • Therapeutic agents targeting tumor cells, including immunotoxins, have shown promise in clinical trials for various cancers.
  • Established tumors and micrometastases are key targets for effective cancer therapies.

Purpose of the Study:

  • To design and characterize novel recombinant gelonin toxin-based immunotoxins.
  • To evaluate the efficacy of these constructs against solid tumors, tumor vasculature, and hematological malignancies.

Main Methods:

  • Fusion of recombinant gelonin toxin with growth factors or single-chain antibodies.
  • In vitro and in vivo characterization of construct cytotoxicity, selectivity, and specificity.

Main Results:

  • Constructs exhibited cytotoxicity at nanomolar or sub-nanomolar levels.
  • Demonstrated impressive selectivity and specificity for antigen-bearing target cells.
  • Successful targeting of solid tumor cells, tumor vasculature, and hematological malignancies.

Conclusions:

  • The designed immunotoxin constructs are potent and selective therapeutic agents.
  • These agents show significant potential as candidates for clinical trials in oncology.
  • Further development of these targeted toxins could lead to improved cancer treatments.

More Related Videos

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo08:13

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo

Published on: January 4, 2012

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Recombinant DNA01:09

Recombinant DNA

Overview