Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 26, 2026

Decellularization of the Murine Cardiopulmonary Complex
08:34

Decellularization of the Murine Cardiopulmonary Complex

Published on: May 30, 2021

Mechanical changes in the rat right ventricle with decellularization.

Colleen Witzenburg1, Ramesh Raghupathy, Stefan M Kren

  • 1Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Journal of Biomechanics
|January 3, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiscale modeling of vascular adaptation: methodological advances and open challenges.

Journal of biomechanics·2026
Same author

Geometric and mechanical changes along the length of the porcine aorta.

Journal of biomechanics·2026
Same author

Alterations in ascending aortic hemodynamics and aortic length correlate with sex-specific thoracic aortic aneurysm dilation and lifespan in a mouse model of severe Marfan syndrome.

Computers in biology and medicine·2026
Same author

Benchtop Pulse Wave Velocity Measurement From Spatial Wavelength Rather Than Pulse Arrival Time: Feasibility Studies.

Journal of biomechanical engineering·2026
Same author

Feasibility of Zero-Dimensional-Model-Based Pulse Waveform Analysis as a Tool to Detect Ascending Thoracic Aortic Aneurysm Growth.

Journal of biomechanical engineering·2026
Same author

Biomechanical Variation of the Vessel Wall Along the Length of the Healthy Aorta-Linking Geometric, Flow-, and Pressure-Mediated Adaptations.

Journal of biomechanical engineering·2025

Decellularized rat ventricles show increased stiffness compared to native tissue, indicating a minor cellular role in stiffness. This study validates decellularized tissue as a model for native extracellular matrix mechanics.

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Cardiovascular Research

Background:

  • Understanding the mechanical properties of the native extracellular matrix (ECM) is crucial for developing effective tissue engineering strategies.
  • Perfusion-decellularization is a common technique to remove cellular components, leaving the ECM scaffold.
  • The mechanical behavior of decellularized cardiac tissue requires further characterization to assess its suitability as a biomaterial.

Purpose of the Study:

  • To compare the stiffness, anisotropy, and heterogeneity of native (control) and perfusion-decellularized rat right ventricles.
  • To evaluate the suitability of decellularized ventricular tissue as a model for native cardiac ECM.
  • To present and validate a novel inverse mechanics method for characterizing soft tissue behavior.

Main Methods:

More Related Videos

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
08:50

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Published on: June 9, 2022

Related Experiment Videos

Last Updated: May 26, 2026

Decellularization of the Murine Cardiopulmonary Complex
08:34

Decellularization of the Murine Cardiopulmonary Complex

Published on: May 30, 2021

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
06:28

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
08:50

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Published on: June 9, 2022

  • Anisotropic inverse mechanics method applied to cruciform rat right ventricle samples.
  • Biaxial loading with simultaneous force measurement and digital image correlation for displacement mapping.
  • Segmentation of tissue into piecewise homogeneous partitions for localized mechanical characterization using a large-deformation elastic model.

Main Results:

  • Perfusion-decellularized rat ventricular tissue exhibited significantly higher stiffness compared to native control tissue.
  • No significant differences in anisotropy or heterogeneity were observed between control and decellularized samples.
  • The cellular contribution to tissue stiffness was found to be relatively small under the tested conditions.

Conclusions:

  • Decellularized rat ventricular tissue, despite quantitative differences in modulus, serves as a viable model of the native tissue ECM.
  • The developed large-deformation inverse method is effective for characterizing complex soft tissue mechanics.
  • Further research can leverage decellularized cardiac ECM for regenerative medicine applications.