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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
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Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Structure01:19

RNA Structure

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Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
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Supramolecular assemblies in functional siRNA delivery: where do we stand?

Hamidreza M Aliabadi1, Breanne Landry, Chongbo Sun

  • 1Department of Chemical & Material Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB, Canada.

Biomaterials
|January 3, 2012
PubMed
Summary

Short interfering RNA (siRNA) therapeutics show promise but require effective delivery carriers. This review explores non-viral carriers, overcoming delivery obstacles, and their potential for clinical applications in RNA interference therapy.

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Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Pharmacology

Background:

  • RNA interference (RNAi) offers therapeutic potential but faces delivery challenges.
  • Short interfering RNA (siRNA) requires carriers for effective delivery and target site action.
  • Non-viral carriers are crucial for assembling siRNA into functional delivery complexes.

Purpose of the Study:

  • To review non-viral approaches for siRNA delivery.
  • To highlight obstacles in siRNA delivery and strategies to overcome them.
  • To present carriers successful in pre-clinical models for potential clinical translation.

Main Methods:

  • Summarizing non-viral siRNA delivery strategies.
  • Analyzing thermodynamic and computational perspectives of supramolecular assembly.
  • Dissecting cellular and systemic delivery/trafficking requirements for siRNA.
  • Reviewing clinical experiences with non-viral siRNA delivery modes.

Main Results:

  • Non-viral carriers are essential for overcoming siRNA delivery barriers.
  • Supramolecular complex formation is key to functional siRNA delivery.
  • Pre-clinical successes offer insights into potential clinical candidates.
  • Engineering approaches address both cellular and systemic delivery challenges.

Conclusions:

  • Non-viral carriers are vital for advancing siRNA therapeutics.
  • Understanding supramolecular dynamics aids in carrier design.
  • Overcoming intracellular and macroscopic barriers is critical for clinical success.
  • Non-viral carriers are poised to significantly impact the future of siRNA therapeutics.