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Updated: May 26, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Rapid genomic-scale analysis of Escherichia coli O104:H4 by using high-resolution alternative methods to
Scott A Jackson1, Michael L Kotewicz, Isha R Patel
1Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, Laurel, MD, USA.
Abstract:
Two technologies, involving DNA microarray and optical mapping, were used to quickly assess gene content and genomic architecture of recent emergent Escherichia coli O104:H4 and related strains. In real-time outbreak investigations, these technologies can provide congruent perspectives on strain, serotype, and pathotype relationships. Our data demonstrated clear discrimination between clinically, temporally, and geographically distinct O104:H4 isolates and rapid characterization of strain differences.
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