Related Experiment Video
Updated: May 26, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
Published on: September 20, 2019
Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis
Nathalie Symens1, Stefaan J Soenen, Joanna Rejman
1Laboratory of General Biochemistry and Physical Pharmacy, Ghent Research Group on Nanomedicines, Ghent University, Ghent, Belgium. symensnathalie@hotmail.com
Abstract:
The nucleocytoplasmic partitioning of nanoparticles as a result of cell division is highly relevant to the field of nonviral gene delivery. We reviewed the literature on the intracellular distribution of cell organelles (the endosomal vesicles, Golgi apparatus, endoplasmic reticulum and nucleus), foreign macromolecules (dextrans and plasmid DNA) and inorganic nanoparticles (gold, quantum dot and iron oxide) during mitosis. For nonviral gene delivery particles (lipid- or polymer-based), indirect proof of nuclear entry during mitosis is provided. We also describe how retroviruses and latent DNA viruses take advantage of mitosis to transfer their viral genome and segregate their episomes into the host daughter nuclei. Based on this knowledge, we propose strategies to improve nonviral gene delivery in dividing cells with the ultimate goal of designing nonviral gene delivery systems that are as efficient as their viral counterparts but non-immunogenic, non-oncogenic and easy and inexpensive to prepare.
Related Concept Videos
Distribution of Cytoplasmic Content
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...
Mitosis and Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Mitosis and Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Mitosis and Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Mitosis And Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Eukaryotic Compartmentalization
For example, lysosomes in the animal cells...

