Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types of Hormones02:13

Types of Hormones

83.7K
Hormones can be classified into three main types based on their chemical structures: steroids, peptides, and amines. Their actions are mediated by the specific receptors they bind to on target cells.
83.7K
Types of Hormones01:21

Types of Hormones

7.2K
Hormones are classified into four main groups: steroids, eicosanoids, amino acid-based derivatives, and peptide hormones.
Steroids and eicosanoids fall under the category of lipid-soluble hormones. Steroids are derived from cholesterol and feature four interconnected carbon rings with variable side chains. Notable examples include estradiol from ovaries and testosterone from testes, exemplifying the critical roles of these lipid-soluble hormones in reproductive physiology. Eicosanoids, derived...
7.2K
Hormonal Regulation01:33

Hormonal Regulation

36.1K
The renin-aldosterone system is an endocrine system which guides the renal absorption of water and electrolytes, thus managing blood pressure and osmoregulation. Activation of the system begins in the kidneys with a small cluster of cells adjacent to the afferent and efferent blood vessels of the renal corpuscle. As the nephrons are filtering blood, juxtaglomerular cells monitor blood pressure. If they detect a decrease in pressure, they release the hormone renin into the bloodstream.
36.1K
Hormonal Regulation01:40

Hormonal Regulation

48.2K
Hormones regulate a significant portion of digestion through activation of the neuroendocrine system. The neuroendocrine system of digestion contains many different hormones all with multiple functions that are both, directly and indirectly, involved in digestion.
48.2K
Plant Hormones01:56

Plant Hormones

27.6K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
27.6K
Measurement: Standard Units03:38

Measurement: Standard Units

80.9K
Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement (a unit), and an indication of the uncertainty of the measurement. While the number and unit are explicitly represented when a quantity is written, the uncertainty is an aspect of the errors in the measurement results.
80.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic Drug Monitoring of Imatinib in Paediatric Chronic Myeloid Leukaemia: Towards Practical Implementation and Interpretation of Measured Plasma Concentrations.

Clinical pharmacokinetics·2026
Same author

An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of 17-hydroxyprogesterone in human serum and plasma.

Clinical chemistry and laboratory medicine·2026
Same author

An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of testosterone in human serum and plasma.

Clinical chemistry and laboratory medicine·2026
Same author

Fontan associated protein-losing enteropathy is linked to distinct metabolic and hepatic alterations.

Scientific reports·2026
Same author

Longitudinal functional lung imaging in children with Post-COVID-19 syndrome.

Molecular and cellular pediatrics·2026
Same author

An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of 5-alpha-dihydrotestosterone in human serum and plasma.

Clinical chemistry and laboratory medicine·2026

Related Experiment Video

Updated: Feb 7, 2026

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

480

Standardized LC-MS/MS based steroid hormone profile-analysis.

Therese Koal1, Diane Schmiederer, Hai Pham-Tuan

  • 1BIOCRATES Life Sciences AG, Innrain 66, A-6020 Innsbruck, Austria. therese.koal@biocrates.com

The Journal of Steroid Biochemistry and Molecular Biology
|January 3, 2012
PubMed
Summary

A new standardized high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay enables accurate quantification of 16 steroid hormones in human serum. This kit-based method offers high performance for clinical diagnostics, improving accuracy for key steroid metabolites.

More Related Videos

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

2.5K
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
09:22

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Published on: October 13, 2017

12.3K

Related Experiment Videos

Last Updated: Feb 7, 2026

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

480
Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

2.5K
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
09:22

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Published on: October 13, 2017

12.3K

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Immunoassays for steroid hormone quantification have limitations.
  • High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) offers potential for improved accuracy.
  • Standardization is crucial for clinical diagnostic assays to minimize variability.

Purpose of the Study:

  • To evaluate a newly standardized HPLC-MS/MS assay in kit format for routine determination of 16 steroid hormones.
  • To assess the assay's performance characteristics in human serum samples.
  • To demonstrate the assay's potential in clinical diagnostics.

Main Methods:

  • A kit-based HPLC-MS/MS assay was developed and standardized for steroid hormone quantification.
  • Sample preparation involved solid-phase extraction (SPE) on a 96-well plate.
  • The assay was validated for human serum, including quantification limits, precision, accuracy, and stability.

Main Results:

  • The assay quantitatively measures 15 steroid hormones, with 11-deoxycorticosterone being semi-quantitative.
  • Validation demonstrated excellent performance, including high linearity (R(2)>0.9966), good precision (intra-day 1.1-8.8%, inter-day 5.2-14.8%), and accuracy (88.3-115.5%).
  • Comparison with existing methods showed improved accuracy for progesterone, androstenedione, and 17α-hydroxyprogesterone.

Conclusions:

  • The standardized HPLC-MS/MS assay provides a high-performance, accurate method for comprehensive steroid hormone profiling in clinical diagnostics.
  • The kit format simplifies routine determination, overcoming limitations of immunoassays.
  • The assay demonstrated potential for analyzing steroid hormone profiles in clinical tests like the metyrapone test.