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Thy-1 (CD90) is an interacting partner for CD97 on activated endothelial cells
Elke Wandel1, Anja Saalbach, Doreen Sittig
1Research Laboratories, Department of Surgery, University of Leipzig, 04103 Leipzig, Germany.
Journal of Immunology (Baltimore, Md. : 1950)
|January 3, 2012
Summary
Leukocyte recruitment involves the interaction between CD97 on myeloid cells and Thy-1 on endothelial cells. This binding is crucial for firm adhesion of polymorphonuclear cells during inflammation.
Area of Science:
- Immunology
- Cell Biology
- Dermatology
Background:
- Leukocyte recruitment to inflammatory sites is vital for immune response.
- Thy-1 (CD90) on activated endothelial cells mediates leukocyte adhesion.
- The role of CD97 in leukocyte-endothelial cell interactions requires further elucidation.
Purpose of the Study:
- To identify novel interacting partners for Thy-1 on endothelial cells.
- To characterize the interaction between CD97 and Thy-1 in leukocyte adhesion.
- To investigate the role of CD97-Thy-1 interaction in inflammatory conditions like psoriasis.
Main Methods:
- Adhesion assays using polymorphonuclear cells (PMNC) and cell lines.
- Protein-binding assays with soluble and immobilized proteins.
- Immunohistochemical analysis of psoriatic skin lesions.
- Blocking studies using monoclonal antibodies (mAbs) against CD97 and Thy-1.
Main Results:
- CD97 was upregulated on PMNC in psoriasis patients and localized with Thy-1 on endothelial cells in skin lesions.
- CD97-expressing cells and soluble CD97 specifically bound to Thy-1.
- The interaction involved the stalk region of CD97 and was calcium-independent.
- Blocking antibodies partially inhibited CD97-Thy-1 binding.
Conclusions:
- CD97 is a novel interacting partner for Thy-1 on activated endothelial cells.
- The CD97-Thy-1 interaction mediates firm adhesion of PMNC during inflammation.
- This interaction may play a significant role in regulating leukocyte trafficking to inflammatory sites.
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