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Hyaluronan in prenodal lymph from skin: changes with lymph flow
R K Reed1, T C Laurent, A E Taylor
1Department of Physiology, University of South Alabama, Mobile 36688.
The American Journal of Physiology
|October 1, 1990
Summary
Skin
Area of Science:
- Dermatology
- Physiology
- Biochemistry
Background:
- Hyaluronan is a key structural component of skin's interstitial matrix.
- Hyaluronan is processed in lymph nodes and the liver.
- Understanding hyaluronan's lymphatic transport is crucial for skin health.
Purpose of the Study:
- To quantify hyaluronan concentration and flux in prenodal lymph.
- To investigate the effect of increased transvascular fluid flux on lymphatic hyaluronan transport.
- To determine the proportion of interstitial hyaluronan removed by lymph.
Main Methods:
- Measurements of lymph flow and hyaluronan concentration in canine hindpaw lymph.
- Stepwise increase in lymph flow induced by elevated venous pressure.
- Analysis of hyaluronan concentration and flux at varying fluid flux rates.
Main Results:
- Lymphatic hyaluronan concentration remained stable until venous pressure exceeded 20 mmHg.
- Hyaluronan concentration decreased at higher venous pressures (around 40 mmHg).
- Lymphatic hyaluronan flux increased significantly with elevated lymph flow, draining more interstitial hyaluronan.
Conclusions:
- A portion of skin hyaluronan is loosely bound and readily cleared by the lymphatic system.
- Increased lymph flow enhances the removal of interstitial hyaluronan.
- These findings provide insights into the dynamic role of lymph in hyaluronan homeostasis in skin.
