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Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals
M Matos Neto1, S S F Santos, M V P Leão
1Basic Science Institute, University of Taubaté, Taubaté, São Paulo, Brazil.
International Endodontic Journal
|January 4, 2012
Summary
All three mechanical preparation systems effectively reduced Enterococcus faecalis in root canals. No significant differences were found in bacterial reduction between the ProTaper rotary, ProTaper manual, and manual K-file systems.
Area of Science:
- Endodontics
- Microbiology
- Dental Materials Science
Background:
- Enterococcus faecalis is a common pathogen in persistent root canal infections.
- Effective mechanical preparation is crucial for reducing intracanal bacterial load.
Purpose of the Study:
- To compare the efficacy of three mechanical preparation systems in reducing Enterococcus faecalis within root canals.
- Evaluate ProTaper rotary, ProTaper manual, and manual K-file systems.
Main Methods:
- Human single-rooted canine teeth were contaminated with E. faecalis and incubated.
- Teeth underwent mechanical preparation using one of the three systems.
- Bacterial samples were collected before and after preparation, cultured, and quantified.
Main Results:
- All tested instrumentation systems significantly reduced Enterococcus faecalis colony-forming units (CFU/mL).
- No statistically significant differences in bacterial reduction were observed among the ProTaper rotary, ProTaper manual, and manual K-file systems.
Conclusions:
- The ProTaper rotary, ProTaper manual, and manual K-file systems demonstrate comparable effectiveness in reducing E. faecalis.
- All evaluated mechanical preparation methods achieve significant bacterial reduction in root canals.
