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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 26, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Minimizing strain influences in a genetically modified mouse phenotyping platform.

Michael D Hayward¹, Olesia Buiakova, David S Grass

¹Taconic, Discovery & Preclinical Research Solutions, Cranbury, New Jersey, USA. Michael.Hayward@Taconic.com

Annals of the New York Academy of Sciences

|January 4, 2012

Summary

This summary is machine-generated.

This study developed a robust research methodology for detecting subtle physiological changes. The approach integrates multiple assays and challenge tests for comprehensive analysis.

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Area of Science:

Biomedical research
Physiology
Experimental design

Background:

Standard research methods may lack sensitivity for detecting minor biological variations.
Integrating diverse assays can enhance the interpretation of complex physiological data.

Purpose of the Study:

To establish a powerful study design for identifying modest physiological alterations.
To improve the cross-functional interpretability of experimental results.
To validate the efficacy of challenge assays in biological research.

Main Methods:

Utilizing appropriately powered studies to detect subtle phenotypic differences.
Implementing assays that assess overlapping physiological systems.
Employing challenge assays to probe system responses under stress.

Main Results:

The methodology enabled the detection of modest changes compared to wild-type controls.
Overlapping assays provided complementary data, enhancing overall interpretation.
Challenge assays successfully elicited measurable physiological responses.

Conclusions:

The integrated approach offers enhanced sensitivity and interpretability for physiological studies.
This methodology is valuable for advancing our understanding of biological systems.
The findings support the use of multi-assay, challenge-based designs in biomedical research.