Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...

Actin Polymerization and Cell Motility

Actin Polymerization and Cell Motility

Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrating synthetic biology to understand and engineer the heart, lung, blood, and sleep systems.

Cell systems·2025

Same author

Microfluidic Production of Ultrathin, Handleable Collagen Sheets Exhibiting Toe-heel Tensile Behavior.

Advanced materials technologies·2025

Same author

Targeting the P-selectin/PSGL-1 pathway: discovery of disease-modifying therapeutics for disorders of thromboinflammation.

Blood vessels, thrombosis & hemostasis·2025

Same author

Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins.

Nature biotechnology·2025

Same author

Synthesis of Sulfonated Peptides Using a Trifluoromethyltoluene-Protected Amino Acid.

The Journal of organic chemistry·2025

Same author

Intact quantitation and evaluation of a PEG-glycosulfopeptide as a therapeutic P-selectin antagonist.

RSC advances·2024

Search research articles

Related Experiment Video

Updated: May 26, 2026

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Published on: August 13, 2021

Cell behavior on a CCN1 functionalized elastin-mimetic protein polymer.

Swathi Ravi¹, Carolyn A Haller, Rory E Sallach

¹Department of Biomedical Engineering, Emory University/Georgia Institute of Technology, Atlanta, GA 30332, United States.

|January 4, 2012

Summary

Researchers developed a new elastin-mimetic polymer, LysB10.V2, that guides endothelial cell functions. This material shows promise for regenerative medicine applications by supporting tissue repair processes.

More Related Videos

Finite Element Modelling of a Cellular Electric Microenvironment

Finite Element Modelling of a Cellular Electric Microenvironment

Published on: May 18, 2021

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

Related Experiment Videos

Last Updated: May 26, 2026

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Published on: August 13, 2021

Finite Element Modelling of a Cellular Electric Microenvironment

Finite Element Modelling of a Cellular Electric Microenvironment

Published on: May 18, 2021

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

Area of Science:

Biomaterials Science
Regenerative Medicine
Cell Biology

Background:

Elastin-mimetic polymers are crucial for tissue engineering.
The matricellular protein CCN1 (cysteine-rich 61, CYR61) plays a vital role in tissue repair.
Engineering materials that mimic natural biological cues is essential for advanced therapies.

Purpose of the Study:

To design an elastin-mimetic triblock copolymer that guides endothelial cell behavior.
To investigate the specific interactions of the V2 ligand sequence with endothelial cells.
To evaluate the potential of this new biomaterial for regenerative medicine.

Main Methods:

Multimerization and cloning of the V2 ligand sequence into the elastin polymer LysB10 to create LysB10.V2.
Cell adhesion, spreading, and migration assays on LysB10.V2 surfaces.
Integrin receptor engagement studies using peptide blocking and focal adhesion analysis.
Haptotactic migration assays to quantify endothelial cell movement.

Main Results:

LysB10.V2 surfaces at a density of 40 pmol/cm(2) promoted endothelial cell attachment.
V2 specifically engaged with the integrin receptor α(v)β(3), leading to focal adhesion assembly.
LysB10.V2 surfaces effectively stimulated endothelial cell migration.
The material supported a quiescent endothelium, indicating potential for controlled biological responses.

Conclusions:

The developed LysB10.V2 polymer successfully mimics elastin while guiding critical endothelial cell functions.
This biomaterial demonstrates significant potential for applications in regenerative medicine, particularly in tissue repair.
Harnessing CCN1's specific functions in biomaterial design offers a promising avenue for advanced scaffold development.