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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Related Experiment Video

Updated: May 26, 2026

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

Pyridine-based lanthanide complexes combining MRI and NIR luminescence activities.

Célia S Bonnet1, Frédéric Buron, Fabien Caillé

  • 1Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans, France.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 4, 2012
PubMed
Summary

Novel triazole-pyridine ligands create versatile lanthanide complexes for advanced medical imaging. These chelating agents offer enhanced properties for both magnetic resonance imaging (MRI) and optical imaging, demonstrating safety and efficacy.

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Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
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Published on: November 10, 2017

Area of Science:

  • Coordination Chemistry
  • Materials Science
  • Medical Imaging

Background:

  • Lanthanide complexes are crucial for medical imaging applications.
  • Developing versatile chelating agents is essential for optimizing both MRI and optical imaging properties.
  • Existing agents often require trade-offs between magnetic resonance (MR) and optical imaging capabilities.

Purpose of the Study:

  • To synthesize novel triazole derivative pyridine-based polyamino-polycarboxylate ligands for lanthanide complexation.
  • To evaluate the suitability of these ligands for both Gd(3+) based MR imaging and near-infrared luminescent lanthanide-based optical imaging.
  • To assess the thermodynamic stability, water exchange properties, and biological safety of the resulting complexes.

Main Methods:

  • Synthesis of novel triazole derivative pyridine-based polyamino-polycarboxylate ligands.
  • Lanthanide complexation and characterization.
  • pH potentiometric measurements for thermodynamic stability constants.
  • Water exchange rate measurements and activation volume determination for Gd(3+) complexes.
  • Optical spectroscopy to assess excitation and emission properties of luminescent lanthanide complexes.
  • In vitro and in vivo toxicity studies.

Main Results:

  • Synthesized ligands form highly stable lanthanide complexes (log K(LnL)=17-19) with selectivity over other metal ions.
  • Gd(3+) complexes exhibit bishydration and faster water exchange rates (k(ex)(298)=7.7-9.3×10(6) s(-1)) with a dissociative mechanism.
  • Luminescent lanthanide complexes show red-shifted excitation energies and increased photon emission due to enhanced epsilon values.
  • The PheTPy ligand system demonstrated particularly low excitation/triplet-state energies and good quantum yields for Nd(3+) and Yb(3+) complexes.
  • Cellular and in vivo studies confirmed the non-toxicity and safety of the bishydrated complexes.

Conclusions:

  • The novel triazole-pyridine ligands provide a versatile platform for simultaneously optimizing MRI and optical imaging properties.
  • These chelating agents offer significant advantages over existing contrast agents for dual-modal imaging.
  • The developed complexes are non-toxic and suitable for in vivo applications, paving the way for advanced biomedical imaging techniques.