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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
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The two faces of His-tag: immune response versus ease of protein purification

Theodore W Randolph1

  • 1Center for Pharmaceutical Biotechnology, Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309, USA. Theodore.Randolph@colorado.edu

Biotechnology Journal
|January 4, 2012
PubMed
Abstract

No abstract available in PubMed .

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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