Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of Recombinant GMPR from <i>Pocillopora damicornis</i> and Potential Mechanisms of Cold-Induced Metabolic Adaptation.

Biology·2026
Same author

Comparative Materials-Level Evaluation of 3'- and 5'-Thiol DNA Aptamer Conjugation on Gold Nanospheres and Nanoflowers: Apparent DNA Loading Output, Morphology Retention, and Qualitative Salt-Challenge Response.

Sensors (Basel, Switzerland)·2026
Same author

A Sea Mud Feed Matrix Shapes Short-Term Dietborne Glyphosate Exposure in the Sea Cucumber (<i>Apostichopus japonicus</i>): Tissue Residues, Buffered Enzyme Responses, and Dominance-Structured Gut Microbiota Shifts.

Animals : an open access journal from MDPI·2026
Same author

Dose-Dependent Intestinal Transcriptomic and Metabolomic Responses to Acute Waterborne Glyphosate Exposure in the Sea Cucumber (<i>Apostichopus japonicus</i>).

Biology·2026
Same author

Emergency call training in primary school children: effects of instruction format and dispatcher influence.

Frontiers in pediatrics·2026
Same author

Physiological Stress Signatures of Waterborne Glyphosate Exposure in <i>Apostichopus japonicus</i>: Insights for Aquatic Ecotoxicology.

Toxics·2026
Same journal

Fecal Proteomics Suggest Potential Biomarkers for Non-Alcoholic Fatty Liver Disease and Steatohepatitis.

Proteomics. Clinical applications·2026
Same journal

An Ensemble Method for Predicting and Designing of Druggable Proteins.

Proteomics. Clinical applications·2026
Same journal

Proteome Profiles of Hypercholesterolemic Patients with and Without FH Treated with a PCSK9 Inhibitor: A Comparison of Depleted and Nondepleted Samples in a Pilot Study.

Proteomics. Clinical applications·2026
Same journal

Proteomic Analysis of Cervicovaginal Fluid for Diagnostic Endometriosis Biomarker Discovery.

Proteomics. Clinical applications·2026
Same journal

Proteomic Analysis of Cerebrospinal Fluid from Severe COVID-19 Patients Reveals Prognostic Biomarkers Associated with Disease Outcome.

Proteomics. Clinical applications·2026
Same journal

A Network-Based Association of IBD and Colorectal Cancer Using Proteomics Data.

Proteomics. Clinical applications·2026
See all related articles

Related Experiment Video

Updated: May 26, 2026

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
10:39

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Published on: August 9, 2022

The current Salmonella-host interactome.

Sylvia Schleker1, Jingchun Sun, Balachandran Raghavan

  • 1Forschungszentrum Jülich, Institute of Complex Systems, Jülich, Germany.

Proteomics. Clinical Applications
|January 4, 2012
PubMed
Summary
This summary is machine-generated.

This study compiles Salmonella-host protein-protein interactions (PPI) to create a gold standard interactome. This resource aids computational models in predicting pathogen-host interactions and understanding Salmonella infection mechanisms.

More Related Videos

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
11:10

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Related Experiment Videos

Last Updated: May 26, 2026

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
10:39

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Published on: August 9, 2022

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
11:10

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Area of Science:

  • Microbiology
  • Proteomics
  • Bioinformatics

Background:

  • Salmonella bacteria cause millions of infections annually, with a broad host range including humans, animals, and plants.
  • During infection, Salmonella utilizes virulence factors and effectors, engaging in protein-protein interactions (PPI) with host cells to facilitate invasion and replication.

Purpose of the Study:

  • To accelerate proteomic research by compiling a comprehensive survey of published Salmonella-host PPI.
  • To establish a gold-standard Salmonella-host interactome for validating computational prediction models.

Main Methods:

  • Conducted a manual literature and database search of over 2200 journal articles and 100 databases.
  • Compiled a list of 62 Salmonella-host PPI, focusing on interactions with human and mouse proteins.
  • Performed pathway analysis using Ingenuity and Broad Gene Set Enrichment Analysis (GSEA) software.

Main Results:

  • Identified a comprehensive Salmonella-host interactome, with only a small fraction of interactions previously available in public PPI databases.
  • Pathway analysis revealed key cellular processes affected by Salmonella, including MAPK signaling, cell death, morphology, and response to infection.
  • The compiled list of 62 PPI represents the most complete Salmonella-host interactome to date.

Conclusions:

  • The curated Salmonella-host PPI list serves as a crucial resource for advancing research on Salmonella pathogenesis.
  • This interactome data is vital for developing and validating computational tools for predicting pathogen-host interactions.
  • Understanding these interactions provides insights into cellular responses to Salmonella and other infectious agents.