Related Experiment Video
Updated: May 26, 2026

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
Published on: January 20, 2023
dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions
Nikolaos Giagtzoglou1, Shinya Yamamoto, Diana Zitserman
1Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA.
Abstract:
Notch signaling governs binary cell fate determination in asymmetrically dividing cells. Through a forward genetic screen we identified the fly homologue of Eps15 homology domain containing protein-binding protein 1 (dEHBP1) as a novel regulator of Notch signaling in asymmetrically dividing cells. dEHBP1 is enriched basally and at the actin-rich interface of pII cells of the external mechanosensory organs, where Notch signaling occurs. Loss of function of dEHBP1 leads to up-regulation of Sanpodo, a regulator of Notch signaling, and aberrant trafficking of the Notch ligand, Delta. Furthermore, Sec15 and Rab11, which have been previously shown to regulate the localization of Delta, physically interact with dEHBP1. We propose that dEHBP1 functions as an adaptor molecule for the exocytosis and recycling of Delta, thereby affecting cell fate decisions in asymmetrically dividing cells.
Related Concept Videos
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...
Recycling Endosomes and Transcytosis
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The Phragmoplast
The...
Distribution of Cytoplasmic Content
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...
Directionality of Nuclear Transport
Microtubule Associated Motor Proteins
