Related Experiment Video
Updated: May 26, 2026
09:27
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
Published on: September 14, 2015
Progenitor/stem cell fate determination: interactive dynamics of cell cycle and microvesicles
Jason M Aliotta1, David Lee, Napoleon Puente
1Division of Hematology and Oncology, Rhode Island Hospital, The Warren Alpert Medical School of Brown University, Providence, RI 02903, USA. jason_aliotta@brown.edu
Stem Cells and Development
|January 5, 2012
Summary
Lung microvesicles alter stem cell behavior, with changes influenced by the stem cell cycle and whether the originating lung tissue was injured. This impacts pulmonary epithelial cell development.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Lung biology
Background:
- Hematopoietic stem/progenitor cell phenotype and differentiation potential vary with the cell cycle.
- Lung-derived microvesicles (LDMVs) can modify marrow cell phenotype, inducing pulmonary epithelial cell-specific gene and protein expression.
- These phenotypic changes are amplified when LDMVs originate from injured lung tissue.
Purpose of the Study:
- To investigate if microvesicle-induced changes in stem/progenitor cell phenotype are linked to the cell cycle.
- To determine if the injury status of the originating lung tissue influences these microvesicle-mediated effects.
- To explore the role of adhesion proteins in microvesicle uptake.
Main Methods:
- Lineage-depleted, Sca-1+ (Lin-/Sca-1+) mouse marrow cells were cultured with cytokines and harvested at different cell cycle phases (G0/G1, late G1/early S, late S/early G2/M).
- These cells were co-cultured with lung tissue, lung-conditioned media (LCM), or LDMVs from irradiated (injured) or non-irradiated mice.
- Alternatively, Lin-/Sca-1+ cells were separated into G0/G1 and S/G2/M phases via fluorescence-activated cell sorting (FACS) without exogenous cytokines for co-culture.
- LDMVs from irradiated and non-irradiated mice were analyzed for adhesion proteins.
Main Results:
- Peak pulmonary epithelial cell-specific mRNA expression occurred in G0/G1 cytokine-cultured cells co-cultured with irradiated lung, and in late G1/early S cells co-cultured with non-irradiated lung.
- Similar patterns were observed when using LCM and LDMVs, and with FACS-separated cells.
- Both cells and LDMVs expressed adhesion proteins, with levels varying based on cell cycle status or radiation injury, suggesting a mechanism for microvesicle entry.
Conclusions:
- Microvesicle-mediated modification of progenitor/stem cells is significantly influenced by the cell cycle stage of the stem cells.
- The treatment or injury status of the originating lung tissue critically affects the properties of the released microvesicles and their impact on stem cells.
- These findings highlight a dynamic interplay between cell cycle, microvesicle origin, and stem cell differentiation potential.
Related Concept Videos
02:36Cells Coordinate Growth and Proliferation
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
02:36Cells Coordinate Growth and Proliferation
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
01:27Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
01:10Zygotic Development And Stem Cell Formation
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
01:21Lineage Commitment
Commitment is the process whereby stem cells:
01:26Stem Cell Niche
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...