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Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: May 26, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Transbilayer phospholipids molecular imaging.

Tarik Z Belhocine1, Frank S Prato

  • 1Department of Medical Imaging, The University of Western Ontario, London, ON, Canada. tbelhocine@yahoo.ca.

EJNMMI Research
|January 5, 2012
PubMed
Summary
This summary is machine-generated.

Nuclear medicine utilizes radiolabeled probes targeting transbilayer phospholipids for molecular imaging. New tracers are advancing preclinical and clinical PET-CT and SPECT-CT imaging of these essential cell membrane components.

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Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

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Last Updated: May 26, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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Published on: October 15, 2016

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Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

Published on: February 17, 2023

Area of Science:

  • Molecular Imaging
  • Cell Biology
  • Radiochemistry

Background:

  • Phospholipid asymmetry is a key feature of mammalian cell membranes.
  • Specific phospholipids reside in either the outer or inner leaflet of the cell membrane.
  • This asymmetry presents unique targeting opportunities.

Purpose of the Study:

  • To review the role of transbilayer phospholipids as targets in molecular imaging.
  • To highlight the development of radiolabeled probes for imaging phospholipid distribution.
  • To discuss applications in positron emission tomography-computed tomography (PET-CT) and single-photon emission computed tomography-CT (SPECT-CT).

Main Methods:

  • Review of current literature on phospholipid distribution and molecular imaging probes.
  • Focus on radiolabeled tracers designed for PET-CT and SPECT-CT.
  • Analysis of preclinical and clinical research findings.

Main Results:

  • Transbilayer phospholipids are validated targets for molecular imaging.
  • Novel radiolabeled tracers enable visualization of phospholipid asymmetry.
  • PET-CT and SPECT-CT are effective modalities for this imaging.

Conclusions:

  • Radiolabeled probes targeting transbilayer phospholipids represent a significant advancement in molecular imaging.
  • The development of these tracers enhances diagnostic capabilities in preclinical and clinical settings.
  • Further research into phospholipid-targeted imaging promises new insights into cellular processes.