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Related Experiment Video

Updated: May 26, 2026

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
08:23

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Published on: September 1, 2013

Cellular and subcellular imaging in live mice using fluorescent proteins.

Robert M Hoffman1

  • 1AntiCancer, Inc., San Diego, CA 92111, USA. all@anticancer.com

Current Pharmaceutical Biotechnology
|January 5, 2012
PubMed
Summary

Fluorescent proteins are revolutionizing in vivo biology imaging. These bright, colorful, and easily manipulated proteins allow real-time observation of cellular processes in live mice, enabling new discoveries in cell biology and drug therapy.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • In Vivo Imaging

Background:

  • Fluorescent proteins (FPs) are essential tools in modern biology.
  • Their properties like brightness, multiple colors, and genetic manipulability make them ideal for in vivo imaging.
  • FPs enable the visualization of biological processes at cellular and subcellular levels.

Purpose of the Study:

  • To review the diverse applications of fluorescent proteins in live animal imaging.
  • To highlight their role in studying various biological phenomena and diseases.
  • To underscore their contribution to the advancement of in vivo cell biology.

Main Methods:

  • Utilizing fluorescent protein-expressing cells for imaging.
  • Employing highly sensitive small animal imaging systems.
  • Observing cellular and subcellular dynamics in real-time within live mice.

Main Results:

  • Demonstrated applications in imaging cancer progression, gene expression, angiogenesis, stem cells, and infections (bacterial, Leishmania).
  • Successful visualization of biological processes at cellular and subcellular levels in live mice.
  • Enabled real-time observation of dynamic biological events.

Conclusions:

  • Fluorescent proteins are pivotal reporters for in vivo imaging across multiple biological disciplines.
  • Real-time imaging in live mice provides novel visual targets for drug development.
  • Fluorescent proteins are foundational to the emerging field of in vivo cell biology.