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Published on: April 12, 2019
Amorphous nanosilicas induce consumptive coagulopathy after systemic exposure
Hiromi Nabeshi1, Tomoaki Yoshikawa, Keigo Matsuyama
1Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
Nanotechnology
|January 5, 2012
Summary
Small nanosilicas (nSP70) cause fatal toxicity and blood clotting issues after systemic exposure. Suppressing their interaction with coagulation factors may prevent these harmful effects, ensuring nanomaterial safety.
Area of Science:
- Nanomaterial safety
- Toxicology
- Biocompatibility
Background:
- Nanosilicas (nSP70) penetrate skin and cause systemic exposure.
- Systemic exposure necessitates understanding nanosilica biological effects.
Purpose of the Study:
- To investigate the biological effects of systemically administered amorphous nanosilicas.
- To determine the relationship between nanosilica size and toxicity.
Main Methods:
- Intravenous injection of amorphous nanosilicas (70, 100, 300, 1000 nm) in BALB/c mice.
- Assessment of survival, blood biochemistry, and coagulation parameters.
- In vitro procoagulant activity assays using factor XII-deficient plasma.
Main Results:
- Injection of 70 nm nanosilicas (nSP70) resulted in fatal toxicity, liver damage, and platelet depletion, indicative of consumptive coagulopathy.
- nSP70 exhibited in vitro procoagulant activity, correlated with increased specific surface area (smaller diameter).
- Procoagulant activity was absent in factor XII-deficient plasma, implicating factor XII in the observed toxicity.
Conclusions:
- The interaction between nSP70 and intrinsic coagulation factors, particularly factor XII, is critical for nSP70-induced toxicity.
- Suppressing this interaction could mitigate harmful effects, enhancing nanomaterial safety.
- Findings provide crucial data for nanomaterial safety assessment and explore new biological applications.
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