Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Antibody interference in immunoassays with markers].

R Sapin1

  • 1Institut de Physique Biologique, Faculté de Médecine, Strasbourg.

Annales De Biologie Clinique
|January 1, 1990
PubMed
Summary

Antibody interferences can affect immunoassay results. This study discusses detecting and mitigating these issues for accurate measurements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[New assays of TSH-receptor antibodies: analytical and clinical performances in the diagnosis of Graves' disease].

Annales de biologie clinique·2009
Same author

Assessing adrenal function in cirrhotic patients: is there a reliable test?

Gastroenterologie clinique et biologique·2009
Same author

Increased reverse triiodothyronine is associated with shorter survival in independently-living elderly: the Alsanut study.

European journal of endocrinology·2008
Same author

[Interferences in immunoassays: Mechanisms and outcomes in endocrinology].

Annales d'endocrinologie·2008
Same author

Efficacy of pioglitazone in familial partial lipodystrophy of the Dunnigan type: a case report.

Diabetes & metabolism·2007
Same author

Etiological diagnosis of hyperprolactinemia.

Annales d'endocrinologie·2007

Area of Science:

  • Immunochemistry
  • Assay development

Context:

  • Immunoassays are widely used for detecting and quantifying analytes.
  • Antibody-based assays are susceptible to various forms of interference.

Purpose:

  • To discuss antibody interferences in immunoassays.
  • To describe the impact of interferences on measured concentrations.
  • To report methods for detecting and eliminating interferences.

Summary:

  • Analyzes antibody interferences in both competitive and non-competitive immunoassays.
  • Considers interferences from analyte-specific antibodies and reagent-specific antibodies (anti-antibodies).
  • Details the effects on apparent measured concentrations and outlines detection and mitigation strategies.

Impact:

  • Improves the reliability of immunoassay results.
  • Provides guidance for assay validation and troubleshooting.
  • Contributes to accurate diagnostic and research applications.

Related Experiment Videos