Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Skeletal Muscle Anatomy00:55

Skeletal Muscle Anatomy

Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Overview of Skeletal Muscle01:15

Overview of Skeletal Muscle

Skeletal muscles are composed of a bundle of muscle fibers and are attached to bones through tendons. Each skeletal muscle fiber is a single muscle cell. The sarcolemma, the plasma membrane of a skeletal muscle cell, consists of a lipid bilayer and glycocalyx that supports muscle fibers. The sarcolemma extends into the muscle cells to form tubular structures called transverse or T-tubules. Each side of the T-tubules consists of a membrane-bound structure called the sarcoplasmic reticulum,...
Microscopic Anatomy of Skeletal Muscles01:13

Microscopic Anatomy of Skeletal Muscles

Skeletal muscle cells, also called muscle fibers, are distinctly elongated, multi-nucleated, slender biological units. They are packed with specialized structures designed to facilitate their primary function, which is contraction.
The muscle sarcolemma is a plasma membrane enclosing each muscle cell that conducts electrical signals called action potentials. The sarcolemma extends into the cell to form T-tubules, ensuring the neural impulses are uniformly distributed across the entire muscle...
Gross Anatomy of Skeletal Muscles01:12

Gross Anatomy of Skeletal Muscles

The connective tissues play a significant role in arranging the muscle fibers into a hierarchical structure that forms a complete muscle. Consider a muscle like the bicep brachii, commonly called the bicep. This muscle comprises thousands of muscle fibers enclosed by a protective layer of connective tissue called the endomysium. The endomysium is primarily composed of reticular fibers, a type of thin collagen fiber. It allows the exchange of nutrients and waste products at the fiber level,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using Cryogenic Electron Tomography (cryoET) to Determine Rubisco Polymerization Constants in α-Carboxysomes.

bioRxiv : the preprint server for biology·2026
Same author

Recent insights into α-carboxysome structure, mechanism, and assembly.

Journal of bacteriology·2026
Same author

Human body temperature cues widespread changes in virulence gene expression in uropathogenic <i>Escherichia coli</i>.

Infection and immunity·2025
Same author

Systematic Characterization of Optical Aberrations Reveals Cryo-FLM Localization Fidelity.

bioRxiv : the preprint server for biology·2025
Same author

Region-Specific Phosphorylation Determines Neuroligin-3 Localization to Excitatory Versus Inhibitory Synapses.

Biological psychiatry·2023
Same author

Effect of Tamoxifen on Proteome Expression during <i>In Vitro</i> Myogenesis in Murine Skeletal Muscle C<sub>2</sub>C<sub>12</sub> Cells.

Journal of proteome research·2023

Related Experiment Video

Updated: May 26, 2026

Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

Skeletal muscle gender dimorphism from proteomics.

Kalina Dimova1, Lauren Ann Metskas, Mohini Kulp

  • 1Center for Proteomics, Smith College.

Journal of Visualized Experiments : Jove
|January 5, 2012
PubMed
Summary

This study presents a reproducible method for analyzing skeletal muscle proteomes, identifying proteins crucial for muscle repair after damage. The technique is applicable to both male and female muscle tissue and other organs.

More Related Videos

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
08:33

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
08:36

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition

Published on: August 31, 2017

Related Experiment Videos

Last Updated: May 26, 2026

Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
08:33

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
08:36

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition

Published on: August 31, 2017

Area of Science:

  • Muscle physiology
  • Proteomics
  • Biochemistry

Background:

  • Skeletal muscle adapts to stressors like exercise and damage.
  • Eccentric contractions can cause muscle damage, requiring protein repair, degradation, and resynthesis.
  • Existing research often overlooks sex-based differences in skeletal muscle adaptation.

Purpose of the Study:

  • To develop a method for identifying proteins involved in skeletal muscle damage repair.
  • To establish a global proteome analysis protocol for differential protein expression.
  • To enable comparative analysis between male and female muscle tissue.

Main Methods:

  • Reproducible protein extraction from male and female muscle.
  • Two-dimensional gel electrophoresis with high-resolution digital imaging.
  • Liquid chromatography-mass spectrometry (LC/MS/MS) for protein identification.

Main Results:

  • A protocol to identify statistically significant (p < 0.05) protein changes (two-fold increase/decrease, appearance/disappearance).
  • Quantification of differential protein expression following muscle stress.
  • Demonstration of applicability to various tissues beyond skeletal muscle.

Conclusions:

  • The presented methodology provides a robust approach to study skeletal muscle proteomic adaptations.
  • This technique aids in identifying key proteins for muscle damage repair and remodeling.
  • The protocol's adaptability allows for broad application in biological research.