Related Experiment Video
Updated: May 26, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Assay to measure oxidized and reduced forms of CoQ by LC-MS/MS
Si Houn Hahn1, Sandra Kerfoot, Valeria Vasta
1Seattle Children's Hospital Research Institute, Seattle, WA, USA. sihahn@uw.edu
Abstract:
The redox status of mitochondrial coenzyme Q (CoQ) is an important marker for oxidative stress associated with several disorders such as Parkinson disease and Alzheimer disease. Altered redox status may be present in mitochondrial electron transport complex disorders. Intracellular CoQ levels reflect the functional status of the mitochondrial electron transport complex better than plasma levels. Here, we describe the method to determine the reduced and oxidized form of CoQ in white blood cells using LC-MS/MS.
Related Concept Videos
Redox Titration: Other Oxidizing and Reducing Agents
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Oxidation and Reduction of Organic Molecules
The removal of an electron from a molecule, results in a...
Redox Titration: Overview
Oxidation-Reduction Reactions
Redox Titration: Iodimetry and Iodometry

