Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 26, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

An integrated on-chip sirtuin assay.

Stefan Ohla1, Reinhild Beyreiss, Gerhard K E Scriba

  • 1Institute of University of Leipzig, Analytical Chemistry, Leipzig, Germany.

Electrophoresis
|January 5, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyclodextrins as Chiral Selectors in Capillary Electrophoresis-Recent Developments in Understanding Analyte Complexation and Applications in Pharmaceutical Analysis.

Electrophoresis·2026
Same author

Development of an ultra-fast polarity-switching ion mobility spectrometer ready for coupling with supercritical fluid chromatography.

Journal of chromatography. A·2025
Same author

Recognition Mechanisms of Chiral Selectors: An Overview.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Cyclodextrins as Chiral Selectors in Capillary Electromigration Techniques.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Application of Molecular Modeling to Capillary Electrophoresis Enantioseparations Promoted by Cyclodextrin-Based Chiral Selectors.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Fast Chemical Analysis of Droplets Unlocked by Ultra-Fast Ion Mobility Spectrometry.

Analytical chemistry·2025

This study presents a novel microchip assay for monitoring human recombinant sirtuin 1 (hSIRT1) activity using a labeled peptide substrate. The developed method enables efficient screening of sirtuin inhibitors.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Microfluidics

Background:

  • Sirtuin 1 (SIRT1) is a crucial enzyme involved in cellular processes.
  • Monitoring SIRT1 enzymatic activity is essential for understanding its role and for drug discovery.
  • Existing methods for assessing SIRT1 activity can be complex or lack efficiency.

Purpose of the Study:

  • To develop a microchip-based assay for monitoring the enzymatic activity of human recombinant sirtuin 1 (hSIRT1).
  • To utilize this assay for screening potential SIRT inhibitors.

Main Methods:

  • A fused silica microchip with integrated serpentine micromixer and microchip electrophoresis was employed.
  • A 9-fluorenylmethoxycarbonyl (Fmoc)-labeled tetrapeptide substrate derived from p53 was used.

More Related Videos

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
08:17

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

Related Experiment Videos

Last Updated: May 26, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
08:17

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

  • Deep UV laser-induced fluorescence detection and dynamic channel coating with hydroxypropylmethyl cellulose were utilized.

    • Main Results:

    • The microchip assay successfully monitored the conversion of the peptide substrate by hSIRT1.
    • Separation of the substrate and deacetylated product was achieved via microchip electrophoresis.
    • The assay demonstrated effectiveness in screening various SIRT inhibitors.

    Conclusions:

    • A sensitive and efficient microchip-based assay for hSIRT1 activity has been developed.
    • This assay is suitable for high-throughput screening of SIRT inhibitors.
    • The microfluidic platform offers a valuable tool for biochemical assays and drug discovery.