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Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
Published on: September 21, 2021
The radioimmunoassay of tricyclic antidepressants
G W Aherne1, E M Piall, V Marks
1Division of Clinical Biochemistry, Department of Biochemistry, University of Surrey, Guildford, Surrey GU2 5XH.
Abstract:
An antiserum to nortriptyline has been produced in a sheep against a nortriptyline-bovine serum albumin conjugate. The conjugate was prepared using the reagent N-(4-bromobutyl) phthalimide followed by a carbodiimide reaction to link the hapten to the carrier protein. The antibodies produced were shown to be specific for the tricyclic group of drugs. The sensitivity of the radioimmunoassay developed is currently limited by the lack of a high specific activity label. The possibilities of developing a radioimmunoassay for nortriptyline in plasma are discussed.
