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Updated: May 26, 2026

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

Published on: December 23, 2011

Isolating LacZ-expressing cells from mouse inner ear tissues using flow cytometry.

Taha A Jan¹, Renjie Chai, Zahra N Sayyid

¹Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, CA, USA.

Journal of Visualized Experiments : Jove

|January 6, 2012

Summary

Researchers developed a novel protocol to isolate LacZ-positive cells from neonatal cochleae using fluorescence-conjugated probes and flow cytometry. This method aids in studying rare stem/progenitor cells in the inner ear, crucial for understanding hearing loss and regeneration.

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Area of Science:

Inner ear biology
Stem cell research
Molecular biology

Background:

Sensorineural hearing loss involves damage to cochlear sensory hair cells.
Murine auditory and vestibular organs may contain stem/progenitor cells with regenerative potential.
Wnt signaling pathway is crucial for maintaining stem/progenitor cells in various organs.

Purpose of the Study:

To develop a method for isolating Wnt-responsive Axin2-expressing cells from neonatal cochleae.
To enable in vitro studies of these cells' behavior as stem/progenitor cells.
To address challenges in studying rare inner ear cell populations.

Main Methods:

Microdissection of neonatal cochlea and tissue dissociation.
Labeling of LacZ-positive cells using a fluorogenic substrate.

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Last Updated: May 26, 2026

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

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Cell isolation via fluorescence-activated cell sorting (flow cytometry).

Main Results:

A modified protocol was established for isolating LacZ-expressing cells from neonatal cochleae.
The protocol enables the analysis of live, isolated Axin2-LacZ positive cells.
This facilitates the interrogation of their stem/progenitor cell characteristics.

Conclusions:

The developed protocol allows for the selective isolation of specific cell types from the neonatal cochlea.
This technique is valuable for studying rare stem/progenitor cell populations in the inner ear.
Further investigation into the regenerative potential of these cells is warranted.