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Colorimetric tracking of protein structural evolution based on the distance-dependent light scattering of embedded

Inhee Choi1, Young In Yang, Eunhye Jeong

  • 1School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul, 151-742, Korea.

Chemical Communications (Cambridge, England)
|January 6, 2012
PubMed

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...