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Updated: Feb 12, 2026

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Published on: October 13, 2019
Regulation of focal adhesion dynamics by Wnt5a signaling
Shinji Matsumoto1, Akira Kikuchi
1Department of Molecular Biology & Biochemistry, Graduate School of Medicine, Faculty of Medicine, Osaka University, Osaka, Japan.
Abstract:
Wnt5a is a representative ligand that activates the β-catenin-independent pathway of Wnt signaling in mammals. This pathway might be related to planar cell polarity signaling in Drosophila. Because reliable biochemical assays to measure Wnt5a pathway activity have not yet been established, we examined whether Wnt5a signaling stimulates focal adhesion turnover in migrating cells using live immunofluorescence imaging and immunocytochemical analysis. These assays demonstrated that the Wnt5a pathway cooperates with integrin signaling to regulate cell migration and adhesion through focal adhesion dynamics.
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