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Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

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Related Experiment Video

Updated: May 26, 2026

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Morphometric analysis of centrosome position in tissues.

Hester Happé1, Emile de Heer, Dorien J M Peters

  • 1Department of Human Genetics and Pathology, Leiden University Medical Center, Leiden, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2012
PubMed
Summary

Planar cell polarity (PCP) is crucial for kidney development. This study introduces a new method to measure centrosome position, serving as a reliable indicator for studying PCP in kidney research.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Renal Physiology

Background:

  • Planar cell polarity (PCP) describes cell polarization within epithelial layers and is vital for tissue development.
  • In the kidney, PCP is hypothesized to regulate tubular diameter, and its disruption is linked to polycystic kidney disease.
  • Studying PCP is essential, but requires robust methodologies.

Purpose of the Study:

  • To establish a reliable method for assessing planar cell polarity (PCP) in biological research.
  • To utilize centrosome positioning as a quantifiable readout for PCP.
  • To facilitate further investigation into the role of PCP in kidney development and disease.

Main Methods:

  • Developed a novel method to measure centrosome position within epithelial cells.
  • Utilized centrosome position as a direct readout for planar cell polarity (PCP).
  • Applied this method to study PCP in the context of kidney research.

Main Results:

  • Successfully established a method to quantify centrosome position.
  • Demonstrated the utility of centrosome position as a readout for planar cell polarity (PCP).
  • Provided a tool to advance the study of PCP in renal physiology.

Conclusions:

  • Centrosome positioning serves as an effective readout for planar cell polarity (PCP).
  • This method offers a valuable tool for researchers studying PCP in kidney development and disease.
  • Further research into PCP's role in kidney function can now be facilitated.