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Updated: May 26, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete nucleotide sequences and genome organization of a cherry isolate of cherry leaf roll virus
Kenneth C Eastwell1, Tefera A Mekuria, Keri L Druffel
1Department of Plant Pathology, Washington State University-I.A.R.E.C., 24106 North Bunn Road, Prosser, WA 99350, USA. keastwell@wsu.edu
Abstract:
The complete nucleotide sequence of cherry leaf roll virus (CLRV, genus Nepovirus) from a naturally infected cherry tree (Prunus avium cv. Bing) in North America was determined. RNA1 and RNA2 consist of 7,893 and 6,492 nucleotides, respectively, plus a poly-(A) tail. Each RNA encodes a single potential open reading frame. The first 657 nucleotides of RNA1 and RNA2 are 99% identical and include the 5'-UTR and the first 214 deduced amino acids of the polyproteins following the first of two in-frame start codons. Phylogenetic analysis reveals close relationships between CLRV and members of subgroup C of the genus Nepovirus.
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