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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
Difference in Th1 and Th17 lymphocyte adhesion to endothelium
Pilar Alcaide1, Elena Maganto-Garcia, Gail Newton
1Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|January 6, 2012
Summary
T helper 17 (Th17) and T helper 1 (Th1) cells show distinct endothelial adhesion, influencing their migration to inflammatory sites. Differences in E-selectin and ICAM-1 interactions explain their unique migratory behaviors.
Area of Science:
- Immunology
- Cell Biology
- Physiology
Background:
- T cell subset migration to inflammation is crucial and endothelium-dependent.
- Distinct migration patterns of T helper 17 (Th17) and T helper 1 (Th1) cells suggest differential adhesion mechanisms.
Purpose of the Study:
- To investigate the adhesion differences between mouse Th17 and Th1 cells to endothelial cells and adhesion molecules.
- To characterize the migratory phenotype of Th17 cells.
Main Methods:
- In vitro adhesion assays of Th17 cells to endothelial adhesion molecules and endothelium under flow.
- In vivo intravital microscopy of Th17 and Th1 cell interactions with microvessels.
- Flow cytometry and quantitative RT-PCR to characterize cell phenotype.
- Air pouch model of inflammation to assess cell recruitment.
Main Results:
- Th17 cells showed greater interaction with E-selectin compared to Th1 cells.
- Th17 cell adhesion to ICAM-1 depended on CCL20-mediated integrin activation.
- CCL20 specifically recruited Th17 cells in an air pouch inflammation model.
Conclusions:
- Endothelial adhesion mechanisms for Th17 and Th1 cells differ quantitatively.
- E-selectin and ICAM-1 play distinct roles in the differential adhesion of Th17 and Th1 cells.
- These adhesion differences contribute to the subset-specific migration of T cells to inflammatory sites.
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